World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Soma ; a Single Oligonucleotide Mutagenesis and Cloning Approach, Volume 8

By Candela, Hector

Click here to view

Book Id: WPLBN0003966892
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Soma ; a Single Oligonucleotide Mutagenesis and Cloning Approach, Volume 8  
Author: Candela, Hector
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Candela, H. (n.d.). Plos One : Soma ; a Single Oligonucleotide Mutagenesis and Cloning Approach, Volume 8. Retrieved from http://nationalpubliclibrary.info/


Description
Description : Modern biology research requires simple techniques for efficient and restriction site-independent modification of genetic material. Classical cloning and mutagenesis strategies are limited by their dependency on restriction sites and the use of complementary primer pairs. Here, we describe the Single Oligonucleotide Mutagenesis and Cloning Approach (SOMA) that is independent of restriction sites and only requires a single mutagenic oligonucleotide to modify a plasmid. We demonstrate the broad application spectrum of SOMA with three examples. First, we present a novel plasmid that in a standardized and rapid fashion can be used as a template for SOMA to generate GFP-reporters. We successfully use such a reporter to assess the in vivo knock-down quality of morpholinos in Xenopus laevis embryos. In a second example, we show how to use a SOMA-based protocol for restriction-site independent cloning to generate chimeric proteins by domain swapping between the two human hRMD5a and hRMD5b isoforms. Last, we show that SOMA simplifies the generation of randomized single-site mutagenized gene libraries. As an example we random-mutagenize a single codon affecting the catalytic activity of the yeast Ssy5 endoprotease and identify a spectrum of tolerated and non-tolerated substitutions. Thus, SOMA represents a highly efficient alternative to classical cloning and mutagenesis strategies.

 

Click To View

Additional Books


  • Plos One : Pesticide Acute Toxicity is a... (by )
  • Plos One : Analysis of Candidate Colitis... (by )
  • Plos One : Influence of Structural Symme... (by )
  • Plos One : Patterns of Spontaneous Brain... (by )
  • Plos One : Association of Chronic Kidney... (by )
  • Plos One : Serum Her 2 Extracellular Dom... (by )
  • Plos One : Dietary Monounsaturated Fatty... (by )
  • Plos One : Development of a Novel Dna Ap... (by )
  • Plos One : Tracking Single Cells in Live... (by )
  • Plos One : the Microrna -23B, Volume 7 (by )
  • Plos One : Cascading Effects of Ocean Ac... (by )
  • Plos One : Comparative Study of Human an... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from National Public Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.